1× mem nonessential amino acids (neaa (Thermo Fisher)

Thermo Fisher is a verified supplier

Thermo Fisher manufactures this product

About

News

Press Release

Team

Advisors

Partners

Contact

Bioz Stars

Bioz vStars

Buy from Supplier

Structured Review

Thermo Fisher 1× mem nonessential amino acids (neaa
1× Mem Nonessential Amino Acids (Neaa, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/1%C3%97+mem+nonessential+amino+acids+(neaa/pm39737939-309-44-49
Average 90 stars, based on 1 article reviews

1× mem nonessential amino acids (neaa - by Bioz Stars, 2026-09

90/100 stars

Images

Knock-Out:

Article Title: Chronic Opioid Treatment Arrests Neurodevelopment and Alters Synaptic Activity in Human Midbrain Organoids.
Article Snippet: .. On day 0, neuronal induction medium composed of 15% Knockout serum replacement (Gibco), 1% GlutaMax (Gibco), 1% minimum essential media-nonessential amino acids (MEM-NEAA) (Gibco), and 0.1% β- mercaptoethanol (Gibco) in Knockout DMEM/F12 (Gibco) supplemented with 100 nm LDN193189 (Stemgent) and 10 μm SB431542 (Tocris Bioscience) was used. .. On day 2, we changed to the neuronal induction media supplemented with LDN193189, SB431542, 100 ng mL−1 SHH (R&D Systems), and 2 μm purmorphamine (Calbiochem).

Cell Culture:

Article Title: SOAT1 dysregulation in astrocytes drives Blood-Brain barrier dysfunction and neuroinflammation in Alzheimer's disease.
Article Snippet: Alzheimer’s disease (AD) is a neurodegenerative disorder that leads to memory loss and cognitive decline, in which blood–brain barrier (BBB) and astrocyte dysfunction are significantly involved.. Recent evidence suggests that dysregulation of lipid metabolism in astrocytes contributes to BBB disruption and neuroinflammation in AD.. Sterol O-acyltransferase 1 (SOAT1), an enzyme involved in cholesterol esterification, has been implicated in BBB disruption and neuroinflammation, but its specific role in AD remains unclear.

Article Title: A novel HDAC8 inhibitor H7E exerts retinoprotective effects against glaucomatous injury via ameliorating aberrant Müller glia activation and oxidative stress.
Article Snippet: The protein–ligand interactions were generated using BIOVIA Discovery Studio Version 21.1.0. (DS Biovia, San Diego, USA). .. The rat retinal Müller cell line rMC-1 and retinal precursor cell line R28 were both purchased from Kerafast (Boston, USA) and rMC-1 was cultured in high glucose Dulbecco’s modified Eagle’s medium (DMEM) (12100046; Gibco, Carlsbad, USA), while R28 was cultured in low glucose DMEM (11885076; Gibco, Carlsbad, USA) containing a 1% solution of minimum essential medium nonessential amino acids (MEM NEAA) (100×) (11140050; Gibco, Carlsbad, USA). .. For general culture, cells were grown in medium supplemented with 10% fetal bovine serum (FBS) and 1% penicillin–streptomycin–glutamine (PSQ) (10378016; Gibco, Carlsbad, USA) in a humidified atmosphere (95% O2 and 5% CO2) at 37 ◦C.

Modification:

Article Title: Distinct chikungunya virus polymerase palm subdomains contribute to viral protein accumulation and virion production
Article Snippet: .. Baby hamster kidney cells (BHK-21 CCL-10, ATCC) were grown in Dulbecco’s Modified Eagle Medium (DMEM, Corning, #10-017-CV) supplemented with 10% fetal bovine serum (FBS, Atlanta Biologicals), 10 mM HEPES (Gibco, #15630080), and 1% nonessential amino acids (MEM-NEAA, Gibco, #11140050). .. Vero cells (CCL-81, ATCC) were grown in DMEM supplemented with 10% newborn calf serum (NBCS, Sigma).

other:

Article Title: Production of fertile XY female animals by silencing of genes on the Y chromosome
Article Snippet: One example of a medium for maintaining pluripotent or totipotent cells in culture, including for example, XY ES cells or XY iPS cells having a reduced capacity to contribute to the male sex determination developmental program after injection into an embryo and intrauterine transfer to a surrogate mother mouse, comprises about 500 ml of base medium in which the following supplements are added: about 90 ml FBS (e.g., Hyclone FBS Cat. No. SH30070.03), about 2.4 millimoles of glutamine (e.g., about 12 ml of a 200 mM glutamine solution, e.g., Invitrogen Cat. No. 25030-081, penicillin:streptomycin (e.g., 60,000 units of Penicillin G sodium and 60 mg of streptomycin sulfate, with about 51 mg of NaCl; e.g., about 6 ml. of Invitrogen pennstrep, Cat. No. 15140-122), about 0.6 millimoles of sodium pyruvate (e.g., 6 ml. of 100 mM sodium pyruvate, Invitrogen Cat. No. 1 1360-070), about 0.06 millimoles of nonessential amino acids (e.g., about 6 ml. of MEM NEAA, e.g., MEM NEAA from Invitrogen Cat. No. 1 1 140-050), about 1.2 ml.

Article Title: Integrin αVβ3 mediates porcine deltacoronavirus infection and inflammatory response through activation of the FAK-PI3K-AKT-nf-κB signalling pathway.
Article Snippet: LLC-PK cells were cultured in Modified Eagle’s medium (MEM, Gibco, USA) supplemented with 5% FBS (Gibco, USA), 1% antibiotic-antimycotic solution, 1% MEM nonessential amino acids (NEAA, Gibco), 1% HEPES (Gibco, USA) in a 5% CO2 incubator at 37 °C.

Review